Service Overview

1

Variant Libraries

Our proprietary technology generates single- and multi-site variants at speed.

MonoPlex
◷ ~1 week

Single-site mutagenesis.
Best for validating targeted mutations. 1 site.

PrecisionPlex
◷ ~2 weeks

Multi-site directed mutagenesis.
Best for exact user-defined combinations across 2–12 sites. 


ComboPlex
◷ ~2 weeks

Combinatorial variant panels.
Best for mapping sequence space at scale. Up to 12 sites.

2

Custom Vector Engineering

For programs that require iterating on the vectors, across coding and non-coding elements, for a specific delivery system or application.

Synthesis, Subcloning,
and Multi-Fragment Assembly

Generation of fragments sourced from de novo synthesis, your existing inventory, or ours, with host-specific codon optimization where needed.


Assembly or subcloning of one or more fragments into new or existing backbones.


Selection and design of assembly strategy.

Backbone Engineering

Customization of the vector backbone, independent of or alongside insert work.


Expression tuning: promoter, UTR, and regulatory element selection for your target host and cell type.


Propagation and selection: Origin and selection marker swaps for bacterial propagation and downstream workflows.

Complex Constructs

Plasmids with inherent assembly challenges: viral vectors (AAV with ITR-flanked cassettes, lentiviral, adenoviral), mRNA and IVT templates (homopolymers), repetitive elements, large inserts, and high-GC regions.

3

External Foundry

Run WayBio as your outsourced DNA foundry. Our modular platform lets you engage at any stage and exit with exactly what you need.

Start from a concept, a sequence, or existing parts already in your inventory

DNA parts assembly and multi-fragment cloning

Automated bacterial transformation, colony screening, and plasmid prep

Scale-up, from research to pre-production quantities

Full plasmid sequence confirmation before delivery

○ Private inventory maintained across all your projects: backbones, inserts, and complete constructs on file

Ready to Build Faster?

Partner with WayBio to accelerate DNA assembly, modification, and validation.

Start a project →